Thursday, August 27, 2020

Budget Essay Example | Topics and Well Written Essays - 1000 words

Spending plan - Essay Example ncreased enthusiasm for research on this theme, and to examine whether the ebb and flow approaches of planning block the viability of current associations. Dynamic Business Environment: The current business condition presents an extremely unique circumstance before the chiefs. In this situation, financial plan is viewed as a requirement as opposed to as an arranging apparatus. Because of this dynamic business condition, the pertinence of the financial plan is extremely fleeting. Moves bring about centralization of the dynamic procedure. This defers the dynamic and lessens and organization’s capacity to react to evolving condition. The idea of how a fruitful organization works in the data age is moving from â€Å"make-and-sell† to â€Å"sense-and respond† (Haeckel, 1999). Planning done in segregation: Many supervisors who are against planning accept that planning energizes a nearsighted arranging skyline showing a delinking of the spending plan and methodology (Shastri, 2008). Financial plans are done in disconnection and are not adjusted to organization technique and objectives. Besides, the planning skyline isn't connected to the business cycle bringing about long planning periods in quickly changing enterprises and short planning periods in very unique ventures. Thwarts Innovation: The organization and controls made because of the planning procedure stop the way of life of stirring things up. The vast majority of the units center around working inside the spending plan consequently lessening the odds of development. The vast majority of the subunits center around working inside their own financial plans and subsequently don't accept development as a way of thinking (Hope and Fraser, 2001). Costly: It is regularly contended that the planning procedure followed at associations is wasteful. This outcomes in the wastage of time of the senior administration. Planning is additionally a costly exercise regarding capital required for the planning reason. Refined Budgeting Techniques: With the downsides of the financial plan and the planning methods,

Friday, August 21, 2020

How To Make Your Prospects Respond To The Webinars

How To Make Your Prospects Respond To The Webinars Make Money Online Queries? Struggling To Get Traffic To Your Blog? Sign Up On (HBB) Forum Now!How To Make Your Prospects Respond To The WebinarsUpdated On 11/05/2019Author : Pradeep KumarTopic : BusinessShort URL : http://hbb.me/2a3fkpa CONNECT WITH HBB ON SOCIAL MEDIA Follow @HellBoundBlogAre you struggling to communicate the value your company delivers? Do your prospects’ eyes gloss over when you start talking about your key differentiators? Open wallets do not wandering eyes make.One of the best ways to focus your prospects’ attention is to offer them something of legitimate value. All the better when that something is legitimately free. Free online webinars, organized and executed by in-house experts whose experience speaks for itself, are a tremendously effective means for doing just that.Make Your Prospects Respond To The WebinarsWhen properly put together, that is. If you’re serious about creating a webinar (or, even better, a webinar series) that does your company pro ud (and does right by its bottom line), you need to make sure it does these four things.1. Put Faces To NamesWell executed online training seminars put company rock stars front and center. Not an overwhelming number of rock stars with poorly defined roles, of course. Just those the seminars’ target audience want and need to hear from. With these names put to faces (or vice versa, take your pick), webinar viewers â€" and, hopefully, newly iced prospects â€" will feel more confident and comfortable reaching out. It’s impossible to quantify the benefit of that confidence.2. Communicate Authority and CompetencySpeaking of confidence, free online training webinars are tailor-made for communicating competency and creating auras of authority. Case in point: this mortgage company’s training page, modest as it is, is authoritative from start to finish. It doesn’t claim to have all the tools necessary to master the mortgage market â€" just a few great tools that you can use to take yo ur real estate business to the next level. You don’t become an authority by promising the world, after all.READTrading Cost - How Stock Brokers Earn From CFD Trades Market?3. Differentiate Your Products Or Services From The Competition’sThat company’s training page isn’t just authoritative. It also clearly and forcefully differentiates its products from the competition’s, without ever naming said competitors. No one likes a sore winner â€" often, it’s best to stand out quietly.4. Create Value-Driven Opportunities For ViewersThe best free online webinars make clear, compelling, high-value offers that reasonable viewers can’t refuse â€" or, at least, have to think long and hard about before refusing. The perception of exclusivity is critical here. Viewers who feel like they’re getting access to a deal that’s not available to anyone else (at least, no one who didn’t watch the same webinar) are more likely to feel that je ne sais quoi connection to the webinar sponso r â€" and more likely to approach their offers with appropriate credulity.The Right Knowledge Really Is PowerThey say knowledge is power, and they’ve been saying it for quite some time. But not all knowledge is created equal. If you’re going to go through the modest but real trouble of putting together high-quality training webinars for your customers and prospects, you need to make sure you’re making a product worthy of your time and effort â€" and your customers’ and prospects’.Just as no one wants to suffer through a tedious lunch-hour meeting that reveals nothing new and establishes no lasting precedents, no one wants to stare blankly at an hour-long webinar only to wonder at the end, “Why did I do that again?”Knowledge really is power â€" when it’s delivered the right way.

Monday, May 25, 2020

Overview of Breast Cancer Essay - 1015 Words

If you or a loved one were diagnosed with breast cancer, how would you feel? You would probably feel very afraid and worried. Breast cancer is a common and potentially deadly form of cancer that affects both men and women. There are risk factors for getting the disease and there are different types of treatment for people who have been diagnosed with the disease. There are also steps that people can take to try to prevent getting breast cancer or at least catch it early. Luckily there are many organizations working to raise awareness of the disease, to educate the public about it, and to support research about it. Breast cancer is a kind of cancer that begins in breast cells. Breast cancer can be labeled as invasive or non-invasive.†¦show more content†¦More than 2.9 million U.S. women with a history of breast cancer were alive on January 1, 2012. There are some known risk factors for developing breast cancer. Women are at a higher risk than men. Older women are at a n increased risk, as most new cases and most deaths occurred in women over the age of 50. There may be a genetic risk for some people; however, most breast cancer is not hereditary. However, if a person has had breast cancer once they are more likely to develop it again. Other risk factors include having very dense breast tissue, beginning menstruating before age 12, going through menopause after age 55, being overweight, using hormone replacement therapy during menopause, not having any children, or having your first child after the age of 35. While there is no absolute way to prevent getting breast cancer, there are some things a woman can do to lower her risk of getting it. First, she should limit the amount of alcohol she drinks. Next, she should not smoke cigarettes. Third, she should lose weight if they need to, as being overweight or obese increases the risk of breast cancer. Fourth, she should exercise regularly. Exercising can help maintain a healthy weight which help s prevent breast cancer. Getting regular medical care and examining her body for symptoms of breast cancer are also important. Women should examine their own breasts for the signs of breast cancer regularly. Women should also have aShow MoreRelatedCell Signaling Essay1583 Words   |  7 Pageshuman disease breast cancer and sickle cell anemia have been the most prevalent. The importance of these topics in reference to breast cancer and sickle cell anemia are the background information on these diseases, the general population affected, what gene/proteins are involved in these diseases, the symptoms of the diseases, the experimental research, and the key advances/ problems relaying to treatment for the diseases. Understanding the background information and how much breast cancer affects peopleRead MoreHuman Epidermal Growth Factor ( Her2 )1153 Words   |  5 Pagesspecialised protein that has the ability to control the growth and spread of certain cancers. Breast cancer is defined as HER2 positive (HER2+) when there is an extremely high level of HER2 receptors present within the cancerous tumour of the breast tissue (Macmillan, 2013). 1.2 Aims and objectives This report aims to give an overview on breast cancer focusing on the HER2+ sub-type of the disease; it includes an overview of aetiology, epidemiology, pathophysiology, clinical presentation, diagnosis andRead MoreBreast Cancer : Causes, Signs And Symptoms And Risk Factors1539 Words   |  7 PagesThis section is going to review literature on overview of breast cancer, its causes, signs and symptoms and risk factors. It will also look at literature on screening methods of breast cancer, particularly mammogram. OVERVIEW OF BREAST CANCER According to Rodney et al (2003), breast cancer is the commonly occurring cancer in women, responsible for one third of all malignancies in females. It is said to be the second to lung cancer as a cause of cancer mortality and has been found to be the leadingRead MoreBreast and Ovarian Cancer Essay1226 Words   |  5 PagesPersuasive Speech Title: Breast and Ovarian Cancer Purpose Statement: This speech has a primary goal of educating the audience and to give a better understanding of what breast cancer is and how it can be treated with detection by a vast variety of methods. It will also give better understanding that not only women but men also are susceptible to breast cancer. Introduction I. Attention: There are a number of researches done about breast and ovarian cancer. Breast cancer is the second largestRead MoreEssay on Breast Cancer Awareness 1413 Words   |  6 PagesAccording to the American Cancer Society, Every year, more than 200,000 women are diagnosed with breast cancer; furthermore Twelve percent of all women will contract the disease, and 3.5% of them will die from breast cancer (American Cancer Society, 2005). There are risk factors that may lead to breast cancer. There are 4 stages of breast cancer and several treatments, although treatments vary from types and stages of breast cancer. Breast cancer is the leading cause of death among women who areRead MoreResearch Paper on Breast Cancer1439 Words   |  6 Pages According to the American Cancer Society, Each year, more than 200,000 women are diagnosed with breast cancer; furthermore Twelve percent of all women will contract the disease, and 3.5% of them will die from breast cancer (American Cancer Society, 2005). There are risk factors that may lead to breast cancer. There are 4 stages of breast cancer and several treatments, although treatments vary from types and stages of breast cancer. Breast cancer is the leading cause of death among women who areRead MoreReviewing The Topic Of Breast Cancer987 Words   |  4 Pagesresearching the topic of Breast Cancer. In this introduction, it will include a problem about the topic and a valid solution in who to solve it. It also discusses the research and research methods put into this technical report, personal qualifications, work schedule, and lastly a table in which lists a time schedule in which this report will be completed. Introduction Breast cancer has a significant amount of impact on the women, men, and families it effects.Breast cancer is only made of topic oneRead MoreIbm Anem Report999 Words   |  4 Pagespurpose of this report is to explore and explain data on male and female breast cancer throughout the United States using IBM Watson Analytics. There are many different topics used throughout the report to describe the impacts of breast cancer, factors that influence breast cancer and ways to prevent the disease. Some of the topics/influences that were used in the charts we created were regional impact, age, race/ethnicity, cancer stage, and mammogram rates. All rates throughout the presentation areRead MoreBreast Cancer Community Resources Essay887 Words   |  4 Pages Breast cancer is the predominant form of cancer diagnosed in America women, second only to skin cancer and the primary cause of cancer deaths, surpassed only by lung cancer. Approximations suggest that one in eight women in the Unite d States will develop invasive breast cancer during the course of their life (American Cancer Society, 2013). As with any disease, a diagnosis of breast cancer can be tremendously challenging and freighting experience not only during the time of treatment and postRead MoreBreast Cancer : Cancer And Cancer1372 Words   |  6 PagesBreast Cancer Disease Overview Breast cancer is a disease in which certain cells in the breast become abnormal and multiply uncontrollably to form a tumor. Breast cancer is the second most commonly diagnosed cancer in women. (Only skin cancer is more common.) About one in eight women in the United States will develop invasive breast cancer in her lifetime. Researchers estimate that more than 230,000 new cases of invasive breast cancer will be diagnosed in U.S. women in 2015. Cancers occur when a

Thursday, May 14, 2020

Definition Essay - The Word Private - 578 Words

Definition Essay - The Word Private Private is a word with a multitude of meanings, some known and others not so known. When searched for in a dictionary, it can be found that the definitions dont vary much between the three dictionaries, although the dictionary from 1913 had a few dissimilar terms. The word private was looked up in Webster Dictionary from 1913, The American Heritage ® Dictionary of the English Language: Fourth Edition, and the Oxford English Dictionary: Second Edition. Many people think of private as meaning something in solitude, or secretive. Reading these dictionary entries revealed the labyrinthine meaning of the word private. Some of the definitions seem very similar to the way private is used in every day†¦show more content†¦Reading these three dictionaries entries raises ones understanding of the word, for the most part, however a couple definitions are found which are not very well-known. The different definitions of the word private basically show that peoples understanding of the word is under construction, even though there are meanings of the word they rarely use. A few of the definitions in the dictionary from 1913 were surprising; e.g. it discusses an archaic definition (see above quote) along with the definition, not general (Private). Among the five pages of entries just on the word private in the Oxford English Dictionary, one definition which was found to be unknown is, Used with reference to medical treatment and facilities for which fees are charged to the patient instead of being provided by the state or a public body (Private). One can learn a lot about the word private after doing a presentation on freedom of expression; when something like that is taught, it is absorbed and remains in the instrcutors memory for some time. The presentation would have to entail things like the USA PATRIOT Act, which has a lot to do with private and privacy matters to be sure. There are a few obscure differences and a few more obvious differences between the three dictionaries. The Oxford English Dictionary is definitely the most complex in its entry on the word private, taking up five pages alone. WebsterShow MoreRelated Definition Essay- The Evolved Meaning of Private657 Words   |  3 PagesDefinition Essay- The Evolved Meaning of Private The word private has changed very much over the past 600 years.   When the word first originated it had a few concise meanings.   Today, the word private has plenty of definitions, some of which many people do not know.   For instance, it is interesting to learn that private means apart from the state or a person not receiving treatment under the National Health Service or any similar state scheme (Landau). Spending time looking through dictionariesRead More Definition Essay – Different Meanings of Private661 Words   |  3 PagesDefinition Essay – Different Meanings of Private â€Å"Property is a central economic institution of any society, and private property is the central institution of a free society.† This was a quote by David Friedman (Friedman). The word dealt with in this essay is ‘private’ and this quote exactly examines one of the prime meanings of private. The main aim of my essay is to explain the different meanings of the word private and to explain the significance and variations of its usage over the pastRead MoreThe Worth of Words951 Words   |  4 PagesThe Worth of Words There is a tacit dichotomy that is found between most words of simple value which inherently have such powerful esteem in what is predominantly a Western society. In many ways, the essay entitled Simple Ways provides numerous examples that support the preceding assertion. The specific terms for and the implicit connotations of words such as good (and its antipode bad), property, and culture, all suggest a polarization that the author presents plentiful evidence to indicate isRead MoreEssay on Check It985 Words   |  4 PagesJuly 28, 2012 English 0310 â€Å"What is Poverty?† Content a. How would you define the author’s purpose? Besides paragraph 15, in what parts of the essay is that purpose most apparent? I would define the author’s purpose is to express the life of a poor person and the views of others towards a poor individual. Besides paragraph 15, Parker’s purpose is most apparent in paragraph two and paragraph six. b. Why does the speaker address her audience directly, especially in paragraphs 4 and 10Read MoreDefinition of Visitor Attraction634 Words   |  3 PagesNo: 40228856 Due on: 14.03.2016, 16:00 Word count: 499 Module leader: Prof A. Leask The purpose of this essay is to further discuss the definition of a visitor attraction. It shall further resolve if events should be defined as visitor attractions, as Swarbrook (2001) characterizes them. Or rather should not be included in the definition as Getz (2008) argues. Visit England (2012) assigns the following characteristics to the definition; the fundamental purpose of an attractionRead MoreMartin Espada And Richard Rodriguezs View Of Bilingualism936 Words   |  4 Pagesbilingualism within their essays. Espada teaches at the University of Massachusetts-Amherst and conducts political poems. In his essay â€Å"The New Bathroom Policy at English High School† he writes about how he thinks English dominates other languages and in the U.S, native English speakers use the dominancy as power to reject other languages. Rodriguez grew up learning Spanish as his first language and attended school at Stanford, Columbia, and University of California. In Rodriguez’s essay â€Å"Hunger of Memory†Read MoreConstructive Criticism and Writing748 Words   |  3 PagesDuring my educational career I have written essays that had purpose or value in an educational environment for grading. Whether this was my intent or not it was what was asked of me by my instructors. Throughout all of these essays I keep a moderate amount of my own honestly throughout them. This is especially so in any essay that must ask questions. Luckily you, the reader, get to witness this. This assignment has asked questions for each and every paragraph that will be included but I might throwRead More The Scope of Woolf’s Feminism in A Room of One’s Own Essay1655 Words   |  7 PagesVirginia Woolf’s extended essay A Room of One’s Own has been repeatedly reviewed, critiqued, and analyzed since its publication in 1929. Arnold Bennett, an early twentieth-century novelist, and David Daiches, a literary critic who wrote an analysis entitled Virginia Woolf in 1942 (Murphy 247), were among those to attempt to extricate the themes and implications of Woolf’s complex essay. The two critics deal with the often-discussed feminist aspect of Woolf’s essay in interestingly differentRead MoreThe Meanings Of A Word By Gloria Naylor984 Words   |  4 PagesThe essay â€Å"The Meanings of a Word† by Gloria Naylor discusses the many definitions of a word and how its meaning can change according to context and delivery. She made this point by telling a story of her childhood and the first time she heard the n-word used by a white person in a derogatory, demeaning way. She described her this situation that took place when she was in third-grade and a boy in front of her in math class called her the n-word. She had no idea what it meant to be called that inRead MoreThe United States Of America s Long Tradition And Definition Of Manliness1686 Words   |  7 PagesDustin Hodgins Professor Vannimwegen English 1A 28, March 2015 What Really Happens to Us The public influences the private to the extent that no one can leave the world’s ideas or thoughts that it throws onto them. Over time, their safe haven of their private space changes and just becomes part of the vast empty world. Men and women can no longer be free to choose the way they want to act; instead the public eye forces them into certain behaviors like gender roles. These roles consist

Wednesday, May 6, 2020

What Is Online Search - 839 Words

you will an find an element that is testable to present a solution to the question. Also, this paper, therefore, offers a simple approach to the question by analyzing the available literature on the same and evaluate their effectiveness in providing an answer. In additional, different libraries are utilized in this research. The online search mechanism is extensively used. Online search employs a number of key phrases and keywords in the search engines. Keywords and phrases used in the search process include; Pick-rounds in player selections, American football player recruitment, Draft position, NBA and NFL success, American football, American basketball, first round draft picks and late round draft picks. Analysis For this test, I†¦show more content†¦Players require environments that are conducive to training and exploration of the talents through fair compensation and rewards as money was discovered as an important factor to motivate players. Players need to feel comfortable in their financial situations to keep up with their celebrity status to facilitate a serein mental capacity for their roles in the field. Rising corrupt practices among officials responsible for the pooling and drafting of players have been linked to poor selection strategies of players in the draft mechanism. The picks from the first rounds do not necessarily stand for the best players to transform the previously low performed clubs. Infiltration of corrupt arrangements ends up making of erroneous drafts that end up drafting players in the wrong round through player’s statistics that are suspicious and unfair. These players may not meet the levels of the productivity expected by the clubs and dropped from the team in a short time. Some players are caught up in their issues due to their fast rise to fame and fail to balance their career and celebrity. A good number of players picked first in the first rounds have displayed to struggle with prejudice as revealed to exist in the industry andShow MoreRelatedOnline Market Is Very Competitive1258 Words   |  6 PagesOnline market is very competitive. There re many websites and blogs fighting for an online audience. To be successful in this highly competitive industry, you require careful planning and execution. More than 75% of the world s population searches for products, services and information through search engines. As online users search for products and services, they type certain keyword phrases that will guide search engines on the information required. Since there re many websites that have similarRead MoreWebsite Development Services : Looking For Professional Website Developer Essay1178 Words   |  5 Pageswell as search engine optimization services so that your website can do well immediately it gets online. Content: The best thing you can do for your business is create a professional website that is able to present your business in an understandable way so that it can help achieve your business goals. It is paramount to work with a company offering professional website development services as well as search engine optimization services so that your website can do well immediately it gets online. OneRead MoreAnalysis Of J. Crews Website1261 Words   |  6 Pageshave put together, or you could have used the search bar that was provided to search for a specific item. Locating desired merchandise: I found that locating the desired merchandise, khaki pants, was a little difficult. Once I navigated to the women’s pants section I found that J. Crew had broken up the pants into the way they fit. I had no way to refine my search on this page. However, when I typed exactly what merchandise I was looking for in the search bar on the home page I was able to find theRead MoreNt1210 Final1163 Words   |  5 PagesNT1210 FINAL EXAM EBOOK INSTITUTE DECEMBER 11, 2014 Nt1210 Final Exam eBook Institute NT1210 FINAL EXAM DOWNLOAD: NT1210 FINAL EXAM Getting Nt1210 Final Exam is easy and simple. Mostly you need to spend much time to search on search engine and doesnt get Nt1210 Final Exam documents that you need. We are here to serve you, so you can easily access, read and download its. No need to wasting time to lookup on another place to get Nt1210 Final Exam. We provide you Nt1210 Final Exam in PDF formatRead MoreSeo Worth : Search Engine Optimization947 Words   |  4 PagesSEO worth Search Engine Optimization, commonly known as SEO, by definition is the process by which a company improves its website’s visibility using paid or unpaid search engine results pages. This process increases the quantity and improves quality of traffic a company earns through search engines. For an SEO tool to be termed as ‘good’, it should be easy to understand, effective in improving your brand, and be fairly priced. In order to find new business opportunities, save time, and keep trackRead MoreOnline Marketing : Marketing Strategy1475 Words   |  6 Pages {Internet Marketing|Online Marketing|Website Marketing|Affiliate Marketing} Gets You More Customers written by: IsaacV {In the current|In the present|In today s|With the current economic} business environment, {it is important|it s important|it is necessary|it is crucial} for entrepreneurs {to adopt|to consider|to look at|to take} new information and technology tools {to help them|to assist them to|to assist them|to enable them to} {carry out|execute|perform|accomplish} business {more efficiently|moreRead MoreEssay about Bbva Compass Case Study854 Words   |  4 Pagesnumber of accounts through online and offline marketing. 1) The role of offline advertising is to build brand awareness and improve consideration among potential customers for opening new checking accounts. The name change of the bank to BBVA Compass dropped the brand awareness to 48% and offline advertising is trying to get it up to 53% by 2010 through sponsoring NBA, WNBA and ESPN. * The role of online advertising is to acquire new customers by creating online brand awareness and openingRead MoreQuestions On Target Discount Code And Save Big1035 Words   |  5 Pagescoupons like Target Discount Code to save online. Assert having use of cutters on store, making buys that seem resilient and assert with web means to trade. Accept use of discount online ascertainments as simple which get you effective run downs with discount buys required. Acquire proper use of valuable cutters on stores making use of discount means to stack with coupons. Make a relative reduction on web acquirement as a remedy in web based uses to acquire online trades. Seek meant cut rate item accessRead MoreSearch Engine Optimization1221 Words   |  5 Pageswww.bambrickmedia.com.au/search-engine-optimisation-australia We’re hear to help you with all your SEO needs. What is Search Engine Optimization? Search engine optimisation is an Internet Marketing method that works to ensure your brand is revealed as high up as possible in the search engines, namely Google. This means it is more visible to those who are searching for your product or service. SEO attempts to optimise your website for the Google algorithms through a variety of techniques that striveRead MoreE Marketing Project1263 Words   |  6 Pageson Flipkart e- marketing tactics covering the following – What are all the eMarketing Tactics that are being used? Please provide proof? †¢ Search Engine Optimization | Pay Per Click Advertising | Social Media Marketing | Banner Advertising | eMail Marketing | Mobile Marketing †¢ What else? – Does FlipKart need each of these tactics? Please give reasons which are related to FlipKart Business, Marketing, Competition, Customers. †¢ Search Engine Optimization | Pay Per Click Advertising | Social Media

Tuesday, May 5, 2020

Physioex Answers free essay sample

Positive and Negative Controls 1. Why are there a number of washing steps in serological tests? The are a number of steps needed in order to remove any non specific binding that may have occurred. 2. Describe how you would know that you had a â€Å"false positive† result. What does this mean for the rest of your results? A positive result with a negative control indicates a â€Å"false positive† and your results are invalidated. 3. Describe how you would know that you had a â€Å"false negative† result.What does this mean for the rest of your results? A negative results with a positive control indicates a â€Å"false negative† and your results are invalidated. Direct Fluorescent Antibody Technique 4. Why is this technique a direct method? It is considered a direct method because it involves conjugation of an antibody with fluorescent dye. 5. What is an elementary body? An elementary body is an infectious particle of any of several microorganisms. We will write a custom essay sample on Physioex Answers or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page 6. How do elementary bodies look under the fluorescent microscope?Elementary bodies look red, no defined varied shape with a darker spot on them and some had green spots or blotches on them. 7. What do you think would happen if you did not fix the sample to the slide with 95% ethyl alcohol? Since ethyl alcohol’s function is to fix the specimen to the slide, I would say the specimen would be washed away during the preparation of the slide if ethyl alcohol was not used. 8. Which patient(s) tested positive for Chlamydia? Patient 2 patient 4 9. Was there any nonspecific binding for any of the samples?Explain. Patient 3 had just a tiny bit of green on it which made me think there was nonspecific binding because there were only two so its not negative or positive but in my opinion human error. Ouchterlony Technique 10. What is a precipitin line? A precipitin line is an insoluble precipitate formed when an antigen and antibody are cross linked. 11. What is the unknown antigen in the simulation? Since there is no reading or text for this activity I don’t understand how I am suppose to figure out what the unknown antigen is. 12.Considering your results, do you think that human serum albumin and bovine serum albumin have epitopes in common? Explain. I would say they have similar or partial epitopes in common but not exact. 13. What is the process resulting in antigen and antibody moving toward each other? One dimensional diffusion Enzyme-Linked Immunosorbent Assay 14. In the â€Å"sandwich† analogy of the direct ELISA, what is the â€Å"bread†? Antibodies What is the filling? Antigens 15. Describe some advantages of using the ELISA technique to process a large number of samples.The advantage of Sandwich ELISA is that the sample does not have to be purified before analysis, and the assay can be very sensitive. 16. Which patient(s) were positive for HIV? Patient C is HIV positive 17. Describe what would happen if you skipped the step where the developing buffer was added. The developing buffer contains the secondary antibodies that the enzyme is conjugated with and without that it wouldn’t develop right and the results would be inconclusive or â€Å"retest†Western Blotting Technique 18. The Western blot technique is used as the confirmatory test for a positive ELISA result because it provides better specificity. How does this technique provide better specificity? The Western blot provides better specificity because it detects antibodies binding to specific proteins and glycoproteins from HIV in the form of bands. 19. If a patient tests positive for HIV using the ELISA test but negative using Western blotting, what can you say about the initial ELISA result?